| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Fibrobacter intestinalis | TGGTGCGGTCATGTCCTTG | AAGCACCACCGCATCCAT | 60.30 | 59.64 | 159 | 113 |
100.00%
|
100.00%
|
| Primer 2 | Fibrobacter intestinalis | GCAAGCGGAATACTGGTGC | AAGCACCACCGCATCCAT | 59.57 | 59.64 | 172 | 113 |
100.00%
|
100.00%
|
| Primer 3 | Fibrobacter intestinalis | GAAGCGACGGGTTACCACA | ACCAGTGCGCAGGGAAAA | 60.01 | 59.81 | 169 | 108 |
100.00%
|
100.00%
|
| Primer 4 | Fibrobacter intestinalis | AAGCGACGGGTTACCACA | ACCAGTGCGCAGGGAAAA | 58.86 | 59.81 | 168 | 108 |
100.00%
|
100.00%
|
| Primer 5 | Fibrobacter intestinalis | AAGCGACGGGTTACCACAC | ACCAGTGCGCAGGGAAAA | 60.30 | 59.81 | 168 | 108 |
100.00%
|
100.00%
|
| Primer 6 | Fibrobacter intestinalis | TGCTCATCGCTTGTGGCA | CGGCTGGATGTCGCTTTTG | 59.97 | 59.86 | 194 | 102 |
100.00%
|
100.00%
|
| Primer 7 | Fibrobacter intestinalis | ATGTTCCGCTTGCGCATG | TTGCCGAAAGCCAGACCTC | 59.51 | 60.30 | 271 | 102 |
100.00%
|
100.00%
|
| Primer 8 | Fibrobacter intestinalis | ACTGCGACCACTTCAAGGC | TGATCGCTTCGCCTTGCA | 60.60 | 60.05 | 171 | 102 |
100.00%
|
100.00%
|
| Primer 9 | Fibrobacter intestinalis | TCCAGGCCAAGGATCACAC | TGATCGCTTCGCCTTGCA | 59.31 | 60.05 | 270 | 102 |
100.00%
|
100.00%
|
| Primer 10 | Fibrobacter intestinalis | GCCGTTCCGCATGCAAAA | ATCCGACTGACCGAGGACA | 60.05 | 60.00 | 179 | 101 |
100.00%
|
100.00%
|